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| Registros recuperados: 18 | |
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| Andrade, Meire Cristina Nogueira de; Unesp/Botucatu; Calonego, Fred Willians; Unesp Botucatu; Minhoni, Marli Teixeira de Almeida; Unesp Botucatu; Severo, Elias Taylor Durgante; Unesp Botucatu; Kopytowski Filho, João; Unesp Botucatu. |
| Avaliou-se o crescimento micelial das linhagens de shiitake LE-95/01, LE- 96/18, LE-96/13, LE-98/55, LE-98/47, LE-95/02, LE-95/06 e LE-95/07, a sua produção em toras de eucalipto e as conseqüentes alterações em características físicas da madeira em decorrência da inoculação com a linhagem LE-96/18. O crescimento micelial foi avaliado em meio de cultura SA (serragem enriquecida com farelo-ágar) a 25ºC. No cultivo em toras, cada linhagem foi inoculada em 40 toras de Eucalyptus grandis com 1 m de comprimento e 9 a 14 cm de diâmetro. As toras foram mantidas em estufa climatizada, com temperatura de 25ºC ±3 e umidade relativa entre 75-90%, durante 6 meses. Em seguida, foi realizado um “choque de indução”, seguido da produção de basidiomas. Verificou-se que: (1)... |
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Palavras-chave: 5.01.02.00-1 Fitossanidade Lentinula edodes; Shiitake; Crescimento micelial; Produção; Toras; Massa específica. |
| Ano: 2007 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/61 |
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| Montini,Renato Mamede de Castro; Passos,José Raimundo de Souza; Eira,Augusto Ferreira da. |
| The mycelium growth kinetics and vigor of shiitake (Lentinula edodes (Berk.) Pegler) strains LE 96/17, LE 98/51, LE 98/53, and LE 98/56 were studied under different agar medium compositions. The strains were from the mycological collection of the Módulo de Cogumelos, Faculdade de Ciências Agronômicas, Unesp-Botucatu, Brazil. Mycelium fragments from stock cultures were transferred to Petri dishes with Sawdust extract-Dextrose-Agar medium. The area of growth and vigor (density) of the mycelia were daily recorded with a digital camera, during incubation, until the complete colonization of the Petri dish. The images were analyzed by the freeware UTHSCSA ImageTool, v. 2.0, developed by the University of Texas Health Science Center, San Antonio. The kinetics of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lentinula edodes; Shiitake; Substrate; Mycelium growth; Strains. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000100017 |
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| Miyaji,CK; Jordão,BQ; Ribeiro,LR; Eira,AF; Cólus,IMS. |
| The mushroom shiitake (Lentinula edodes (Berkeley) Pegler) is been widely consumed in many countries, including Brazil, because of its pleasant flavor and reports of its therapeutic properties, although there is little available information on the genotoxicity and/or antigenotoxicity of this mushroom. We used the Comet assay and HEp-2 cells to evaluate the in vitro genotoxic and antigenotoxic activity of aqueous extracts of shiitake prepared in three different concentrations (0.5, 1.0 and 1.5 mg/mL) and three different temperatures (4, 22 and 60 °C), using methyl methanesulfonate (MMS) as a positive control and untreated cells as a negative control. Two concentrations (1.0 and 1.5 mg/mL) of extract prepared at 4 °C and all of the concentrations prepared at... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Shiitake; Comet assay; HEp-2 cells; Genotoxicity; Antigenotoxicity. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100018 |
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| Regina,Magali; Broetto,Fernando; Giovannozzi-Sermanni,Giovanni; Marabotini,Rosita; Peranni,Claudio. |
| This work aimed to study the stationary and periodically mixed culture of L. edodes to the production of lignocellulolitic enzymes activity. LE 95/17, LE 96/22 and Leax strains were incubated in 25 g of eucalyptus sawdust substrate in Erlenmeyer flasks in stationary culture at 25º C and in a bioreactor with four complete rotations daily at 25º C and 3% CO2. The samples were collected at 8, 11, 14, 17 and 20 days after the incubation. Oxidative and hydrolytic enzymes analyses were performed. Lignin peroxidase enzyme was not found in the lignolytic system for LE 95/17, LE 96/22 and Leax strains in the different incubation methods. The use of bioreactor could be a practicable system to induce the laccase activity for L22 and Leax and MnP activity for L17 and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Shiitake; Enzymes; Agricultural residues. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200001 |
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| Di Piero,Robson M.; Wulff,Nelson A.; Pascholati,Sérgio F.. |
| The shiitake mushroom (Lentinula edodes) has been used in research involving the prevention and the control of human and plant diseases. In cucumber plants, treated with aqueous extracts from mushroom basidiocarps, there was a reduction in anthracnose severity caused by Colletotrichum lagenarium, and an increase in peroxidase activity in the leaves. With the aim of obtaining molecules of agronomic interest, the crude aqueous extract from L. edodes basidiocarp was fractioned with ammonium sulfate. The fraction corresponding to 40-80% of saturation (p40-80), the most effective in reducing anthracnose on cucumber cotyledons, was submitted to anion exchange chromatography (AEC). After AEC, six protein peaks were obtained and the peak V, containing 34% of the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Shiitake; Anthracnose; Peroxidase. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000200015 |
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| Cavallazzi,José Renato P.; Kasuya,Catarina M.; Soares,Marcos A.. |
| Laccases are enzymes involved in lignin degradation and are produced by various organisms. Due to their low substrate specificity their potential to be used in biotechnological applications has received attention. The addition of laccase inducers to the culture medium of microorganisms can enhance laccase production and facilitate its purification and utilization. The aim of this study was to investigate the effect of some compounds as laccase inducers in cultures of Lentinula edodes (shiitake). First, it was selected a culture medium suitable for laccase production by shiitake using two levels of N (2.6 and 26 mM) and seven levels of Cu (0, 50, 100, 150, 200, 250 and 300 µM). The medium with 2.6 mM N and 250 µM Cu was found to provide the highest laccase... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Laccase; Shiitake; Laccase inducers. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400015 |
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| Rossi,Ivan Henrique; Monteiro,Antonio Carlos; Machado,José Octavio; Andrioli,João Luciano; Barbosa,José Carlos. |
| This investigation was performed to evaluate the biological efficiency (BE), mean mushroom weight (MMW), mean number of mushroom (MNM) and mushroom quality of Shiitake [ Lentinula edodes (Berk.) Pegler] when grown on a sterilized substrate composed by sugarcane bagasse enriched with rice bran and sugarcane molasses. The proportions of rice bran were 0, 15, 20, 25 and 30% (dry weight/dry weight of bagasse); and the concentrations of sugarcane molasses were 0, 30 and 60 g/kg (dry weight/dry weight of bagasse plus rice bran). Four flushes were obtained during the production cycle, providing 3 accumulated productions which were used for production analysis. The substrate supplemented with 25 and 30% rice bran yielded the highest BE (98.42 and 99.84%,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lentinula edodes; Shiitake; Sugarcane bagasse. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100014 |
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| Sasaki,Selma H.; Linhares,Rosa E.C.; Nozawa,Carlos M.; Montalván,Ricardo; Paccola-Meirelles,Luzia D.. |
| Four Lentinula edodes strains (Le10, 46, K2, Assai) were assessed for their antagonistic effect on four filamentous fungus species of agricultural importance (Helminthosporium euphorbiae, Helminthosporium sp, Fusarium solani and Phomopsis sojae) and on Alagoas serotype of Vesicular Stomatitis Virus (VSA). The L. edodes strains studied had variable effects on the filamentous fungi and on VSA. The K2 and Le10 strains were antagonistic on the fungi assessed and the 46 and K2 strains were efficient on the Vesicular Stomatitis Virus. The results widened the list of beneficial effects of L. edodes on the control and prevention of animal pathogenic virus and filamentous fungi. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phytopathogenic fungi; Shiitake; Inhibition; Vesicular Stomatitis Virus (Alagoas). |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100012 |
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| Rossi,Ivan Henrique; Monteiro,Antonio Carlos; Machado,José Octavio; Barbosa,José Carlos. |
| The purpose of this study was to assess the myceliation rate, mycelial vigor and "estimated biomass" of Lentinula edodes (Berk.) Pegler, grown on a sugarcane bagasse substrate enriched with rice bran and sugarcane molasses for spawn production. The proportions of rice bran used were 0, 10, 15, 20, 25, 30 and 40% (dry weight/dry weight of bagasse) and the sugarcane molasses concentrations tested were 0, 10, 20, 30, 40, 50 and 60 g/kg (dry weight/dry weight of bagasse plus rice bran). The myceliation rate was decreased by the addition of the higher quantities of rice bran. The 25 and 30% rice bran proportions induced the highest stimulation of mycelial vigor. The addition of sugarcane molasses did not change myceliation rate or mycelial vigor. The "estimated... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lentinula edodes; Shiitake; Sugarcane bagasse. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100013 |
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| Shiomi,Humberto Franco; Minhoni,Marli Teixeira de Almeida; Machado,José Octávio; Cargnelutti Filho,Alberto. |
| The aim of this work was to evaluate the effect of thermal and mechanical shocks on the productivity of Lentinula edodes colonized on 140 Eucalyptus saligna logs at different immersion times in water and in the first flush of production. The logs were immersed in water at low temperature (16ºC) or environmental temperature (22ºC), for 6, 10, 14, 18, 22, 26 and 38 hours. The mechanical shock treatment was accomplished by dropping the logs onto the floor three consecutive times, in a vertical position. Water temperature and immersion time affected L. edodes yield, which increased two to four times when the logs were immersed in cool water for the shortest times (6 and 10 hours). The mechanical shock treatment did not increase the sporophore yield. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Shiitake; Stress factors; Lentinula edodes; Sporophore yield. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200003 |
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| Paula,Débora Pires; Tarsitano,Maria Aparecida Anselmo; Graciolli,Luiz Antônio. |
| O interesse nacional pelo cultivo comercial de shiitake é crescente, devido às suas propriedades nutricionais e medicinais, e ao seu apreciável sabor. O cultivo deste cogumelo constitui uma alternativa rentável de diversificação das atividades de uma propriedade agrícola. Este trabalho teve como objetivo determinar, no Estado de São Paulo, a viabilidade econômica do cultivo de shiitake considerando-se um ciclo de 14 meses, em 1000, 2000, 3000 e 4000 toros de eucalipto. As escalas de produção de 2000 e 4000 toras apresentaram os menores custos de produção kg-1 de shiitake fresco, cerca de R$6,50 e os melhores índices de lucratividade, aproximadamente 34%. A análise de viabilidade econômica mostrou resultados favoráveis para as três maiores escalas de... |
| Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Lentinula edodes; Cogumelo; Shiitake; Viabilidade econômica. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162001000200031 |
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| Registros recuperados: 18 | |
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